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Image Search Results
Journal: bioRxiv
Article Title: Reverse engineering of a pathogenic antibody reveals the molecular mechanism of vaccine-induced immune thrombotic thrombocytopenia
doi: 10.1101/2023.06.30.547300
Figure Lengend Snippet: Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured Protein A chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.
Article Snippet: The
Techniques: SPR Assay, Binding Assay, Concentration Assay
Journal: Cell Communication and Signaling : CCS
Article Title: Impairment of IGF2 gene expression in prostate cancer is triggered by epigenetic dysregulation of IGF2 -DMR0 and its interaction with KLF4
doi: 10.1186/s12964-017-0197-7
Figure Lengend Snippet: Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP agarose gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test
Article Snippet: After blocking of unspecific DNA with salmon sperm DNA for 1 hour at 4 °C, the sonicated nuclear material was immunoprecipitated using specific histone antibodies (anti-H3K27me3, −H3K9me3, and –H3K4me3 from Abcam, and anti-KLF4 from R&D Systems) by adding the
Techniques: Expressing, DNA Methylation Assay, Generated, Agarose Gel Electrophoresis, Methylation, MANN-WHITNEY