staphylococcal protein a-conjugated colloidal gold particles Search Results


90
XanTec bioanalytics the protein a conjugated chip
Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured <t>Protein</t> <t>A</t> chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.
The Protein A Conjugated Chip, supplied by XanTec bioanalytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher protein a–conjugated dynabeads
Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured <t>Protein</t> <t>A</t> chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.
Protein A–Conjugated Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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EY Laboratories protein a conjugated gold colloidal particles-20
Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured <t>Protein</t> <t>A</t> chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.
Protein A Conjugated Gold Colloidal Particles 20, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pharmacia LKB Biotechnology Inc protein-a conjugated beads
Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured <t>Protein</t> <t>A</t> chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.
Protein A Conjugated Beads, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Thermo Fisher protein aconjugated agarose beads
Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured <t>Protein</t> <t>A</t> chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.
Protein Aconjugated Agarose Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cytiva Europe protein a hrp
Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured <t>Protein</t> <t>A</t> chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.
Protein A Hrp, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc protein a conjugated sepharose
Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP <t>agarose</t> gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test
Protein A Conjugated Sepharose, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc protein a hrp conjugate
Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP <t>agarose</t> gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test
Protein A Hrp Conjugate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology protein a conjugated agarose beads
Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP <t>agarose</t> gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test
Protein A Conjugated Agarose Beads, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc protein a conjugated sepharose 4b
Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP <t>agarose</t> gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test
Protein A Conjugated Sepharose 4b, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Thermo Fisher protein a conjugated horseradish peroxidase
Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP <t>agarose</t> gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test
Protein A Conjugated Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher dynabeads protein a-conjugated magnetic beads
Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP <t>agarose</t> gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test
Dynabeads Protein A Conjugated Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured Protein A chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.

Journal: bioRxiv

Article Title: Reverse engineering of a pathogenic antibody reveals the molecular mechanism of vaccine-induced immune thrombotic thrombocytopenia

doi: 10.1101/2023.06.30.547300

Figure Lengend Snippet: Surface-plasmon resonance sensograms obtained for the binding of the PF4 to the RVT1-captured Protein A chip. The concentration of the PF4 was titrated from 100nM till 2uM concentrations, and the signal was normalized to the sensograms with the blank solution whose composition matches the solution of PF4.

Article Snippet: The Protein A conjugated chip was acquired from Xantec Ltd (Düsseldorf, Germany).

Techniques: SPR Assay, Binding Assay, Concentration Assay

Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP agarose gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test

Journal: Cell Communication and Signaling : CCS

Article Title: Impairment of IGF2 gene expression in prostate cancer is triggered by epigenetic dysregulation of IGF2 -DMR0 and its interaction with KLF4

doi: 10.1186/s12964-017-0197-7

Figure Lengend Snippet: Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2 -DMR0 and IGF2/H19 -ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI , GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP agarose gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2 -mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2 -DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19 -ICR was found in LOI-group. P-values were determined by Mann-Whitney U test

Article Snippet: After blocking of unspecific DNA with salmon sperm DNA for 1 hour at 4 °C, the sonicated nuclear material was immunoprecipitated using specific histone antibodies (anti-H3K27me3, −H3K9me3, and –H3K4me3 from Abcam, and anti-KLF4 from R&D Systems) by adding the protein A-conjugated Sepharose (Amersham Biosciences and GE Biosciences).

Techniques: Expressing, DNA Methylation Assay, Generated, Agarose Gel Electrophoresis, Methylation, MANN-WHITNEY